A novel method for evaluating antibody-dependent cell-mediated cytotoxicity by flowcytometry using cryopreserved human peripheral blood mononuclear cells

A novel method for evaluating antibody-dependent cell-mediated cytotoxicity by flowcytometry using cryopreserved human peripheral blood mononuclear cells.

Analyzing the cytotoxic functions of effector cells, such as NK cells against target cancer cells, is thought to be necessary for predicting the clinical efficacy of antibody-dependent cellular cytotoxicity (ADCC) -dependent antibody therapy. The (51)Cr release assay has long been the most widely used method for quantification of ADCC activity. However, the reproducibilities of these release as...

Antibody-Dependent Cellular Cytotoxicity of Within Peripheral Blood Mononuclear Cells,

Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

Human monocyte antibody-dependent cell-mediated cytotoxicity to tumor cells.

Previous investigations of mononuclear cell antibody-dependent cell-mediated cytotoxicity (ADCC) toward tumor cells suggest that K lymphocytes and not monocytes are active in this cytotoxic reaction. This report, however, demonstrates that human monocytes are able to carry out ADCC toward three different human tumor cell lines (CEM T lymphoblasts, Raji bone marrow-derived (B) lymphoblasts, and ...

Human lymphocyte antibody-dependent cell-mediated cytotoxicity (ADCC) toward human red blood cells.

Previous investigations involving mononuclear cell antibody-dependent cellmediated cytotoxicity (ADCC) toward human red cell (RBC) targets indicated that monocytes but not lymphocytes are able to produce erythrocyte lysis. We reexamined this reported insensitivity of human RBC to lymphocyte cytotoxicity using a variety of human RBC targets and a wide range of antisera to various erythrocyte ant...